Anti-FOXA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P55317

Core Product Specifications and Parameters

Biological Background: FOXA1 Function and Localization

• FOXA1 (Forkhead box protein A1; HNF3A; TCF3A) is a pioneer transcription factor that regulates gene expression by opening chromatin and recruiting other transcription factors to enhancer/promoter regions.
• Binds DNA via a conserved forkhead domain recognizing the consensus 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' and facilitates cell-type-specific transcriptional programs dependent on histone modifications such as H3K4me2.
• Essential for embryonic development of endoderm-derived organs including the liver, lung, prostate, and pancreas; cooperates with FOXA2 in these processes.
• Modulates nuclear hormone receptor signaling, critically required for ESR1 (estrogen receptor) binding to target promoters in breast cancer and for estrogen-dependent gene regulation.
• Controls cell survival and proliferation by repressing BCL2 (anti-apoptotic) and activating CDKN1B (p27/Kip1 cell cycle inhibitor), partly through interaction with BRCA1.
• Activates transcription of liver-specific genes (albumin, AFP, PEPCK) and contributes to metabolic homeostasis including glucose regulation.
• Found predominantly in the nucleus; highly expressed in prostate, ESR1-positive breast tumors, and overexpressed in lung and esophageal adenocarcinomas.
• Undergoes alternative splicing and phosphorylation, consistent with its regulation at multiple levels.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 263-472) of human FOXA1, which includes part of the forkhead domain and transactivation domain; this region is highly conserved across human, mouse, and rat, supporting cross-reactivity.
• For WB, a band near 49 kDa is expected; consider using nuclear extracts from FOXA1-expressing tissues or cell lines (e.g., liver, prostate, breast cancer) and validate antibody specificity with appropriate controls.
• IHC-P may require antigen retrieval and optimization of antibody dilution; nuclear staining is anticipated. Validate on positive control tissues such as human liver or prostate adenocarcinoma.
• For ELISA, the antibody can be tested as a capture or detection reagent; pairing with a monoclonal anti-FOXA1 antibody may improve sandwich assay performance after in-house validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We support transcription factor and cancer biomarker research by sourcing rare-target antibodies like anti-FOXA1 and offering customized solutions for assay development. Our one-stop supply chain covers every stage from concept to clinic, ensuring reliable and scalable procurement.