Anti-FOSL2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P15408

Core Product Specifications and Parameters

Biological Background: Fos-related antigen 2 Function and Localization

• FOSL2 (FRA2), a member of the Fos family of transcription factors, forms heterodimers with JUN proteins to regulate gene expression.
• Involved in osteoclast biology: controls osteoclast survival and size (By similarity).
• Mediates PGE2-induced osteoblast activity by activating CEBPB transcription (By similarity).
• Forms a heterodimer with JUN to activate LIF gene transcription (By similarity).
• Subcellularly localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Post-translational modifications include acetylation and phosphorylation, and it undergoes ubiquitin conjugation.
• Alternative splicing generates multiple isoforms; mutations in FOSL2 are associated with ectodermal dysplasia.
• Keywords: Acetylation, Alternative splicing, DNA-binding, Nucleus, Phosphoprotein, Transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• For Western blot, consider the predicted molecular weight of approximately 35 kDa; validate detection in relevant cell lines or tissues expressing FOSL2.
• In immunohistochemistry (paraffin) and immunofluorescence/ICC, expect nuclear localization; optimize fixation and permeabilization protocols to ensure antibody accessibility.
• As a polyclonal antibody raised against a recombinant fragment, it may recognize multiple FOSL2 isoforms; confirm specificity for your target isoform if alternative splicing is a concern.
• For ELISA applications, pair with appropriate secondary antibodies and validate in the desired sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For transcription factor research and bone biology, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies like anti-FOSL2, bulk ancillary reagents, and can source rare-target raw materials. Partner with us to streamline your IVD development pipeline.