Anti-FOSB Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P53539

Core Product Specifications and Parameters

Biological Background: FOSB Function and Localization

• FOSB encodes the protein FosB, a member of the AP-1 transcription factor complex subunit family, also referred to as G0S3, GOS3, GOSB, and FOSB.
• Heterodimerizes with JUN family proteins to form the AP-1 transcription factor complex, enhancing DNA binding to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and promoting transcriptional activity. Related references: PMID:12618758 PMID:28981703
• As part of the AP-1 complex, facilitates enhancer selection by cooperatively binding to nucleosomal enhancers and recruiting the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin (By similarity).
• Together with JUN, participates in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L and inducing its transcription in response to TCR/CD3 signaling. Related references: PMID:12618758
• Alternative splicing produces isoforms; isoform 2 shows lower transactivation activity than isoform 1 and forms an inactive heterodimer with JUN, potentially acting as a transcriptional inhibitor (By similarity).
• Subcellular location is primarily nuclear, consistent with its role as a transcription factor.
• Tissue specificity includes expression in the nucleus accumbens of the striatum (at protein level).

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against an N-terminal synthetic peptide (amino acids 1-100) of human FOSB; sequence homology with mouse and rat FOSB may account for the observed cross-reactivity.
• In western blot, a band near 36 kDa is expected; however, additional bands or shifts may occur due to post-translational modifications or isoform expression, warranting validation in the relevant sample system.
• For immunohistochemistry (paraffin-embedded sections), nuclear staining should be observed based on FOSB localization; optimize antigen retrieval and primary antibody dilution for each tissue type.
• In ELISA, consider employing the antibody as either a capture or detection reagent, with appropriate controls to ensure specificity.
• To confirm binding specificity, a peptide competition assay using the immunogen peptide is recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material procurement partner, CamelBio streamlines access to antibodies, proteins, and ancillary reagents for neuroscience and oncology research. Our product portfolio includes validated antibody pairs, recombinant proteins, and bulk ancillary reagents to support assay development from concept to clinic. For researchers studying AP-1 transcription factor pathways in neurogenesis, apoptosis, or addiction, we offer one-stop sourcing of FOSB antibodies and related pathway reagents, enabling seamless integration into your diagnostic workflow.