Anti-FOS Rabbit Polyclonal Antibody for WB, ELISA - P01100

Core Product Specifications and Parameters

Biological Background: c-Fos Function and Localization

• Encodes a nuclear phosphoprotein that forms a tight non-covalent heterodimer with JUN/AP-1 transcription factor, binding to symmetrical DNA half sites to regulate gene expression.
• On TGF-beta activation, assembles into a multimeric SMAD3/SMAD4/JUN/FOS complex at AP1/SMAD-binding sites, mediating TGF-beta signaling.
• Plays a critical role in skeletal development, maintaining cells destined to form and maintain the skeleton.
• Involved in signal transduction, cell proliferation, and differentiation.
• In growing cells, activates phospholipid synthesis by possibly activating CDS1 and PI4K2A, requiring Tyr-dephosphorylation and association with the endoplasmic reticulum.
• Localizes to the nucleus, endoplasmic reticulum, cytoplasm, and cytosol.
• Identified as a proto-oncogene; subjected to alternative splicing, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human FOS, suggesting the antibody may detect multiple isoforms expressed through alternative splicing.
• For western blot, consider using whole cell lysates from serum-stimulated or growth factor-treated cell lines to upregulate FOS expression; the predicted molecular weight is approximately 41 kDa.
• In ELISA, the peptide-based immunogen may enable detection of c-Fos in denatured or native lysates; include appropriate peptide or recombinant standards for quantification.
• Cross-reactivity with mouse and rat indicates conserved epitope; however, validate in the desired species before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a one-stop platform for IVD raw material procurement, technical support, and consulting services from concept to clinical application. This anti-FOS polyclonal antibody targets a pivotal transcription factor in oncology and cell differentiation studies. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can help source rare-target raw materials to accelerate your diagnostic assay development.