Anti-Fos B Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P53539

Core Product Specifications and Parameters

Biological Background: Fos B Function and Localization

• Protein Fos B is a member of the Fos family of transcription factors and heterodimerizes with JUN family proteins to form the AP-1 transcription factor complex, which binds to 5'-TGA[GC]TCA-3' consensus sequences on gene promoters (PubMed:12618758, PubMed:28981703). Related references: PMID:12618758, PMID:28981703

• As part of the AP-1 complex, Fos B facilitates enhancer selection by collaborating with cell-type-specific transcription factors, binding to nucleosomal enhancers, and recruiting the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin (By similarity).

• Fos B, together with JUN, regulates activation-induced cell death in T cells by binding to the AP-1 site in the FASLG/CD95L promoter and inducing its transcription upon TCR/CD3 signaling (PubMed:12618758). Related references: PMID:12618758

• In the brain, Fos B is expressed in the nucleus accumbens and is implicated in neurogenesis, learning, memory, and behavioral responses to drug reward and spatial memory (By similarity). It also plays a role in synaptic modifications in medium spiny neurons and adaptive reward-dependent learning (By similarity).

• An alternatively spliced isoform (isoform 2) exhibits lower transactivation activity and can act as a transcriptional inhibitor when heterodimerized with JUN; it has a longer half-life (~9.5 hours) compared to isoform 1 (~1.5 hours) (By similarity).

• Subcellularly, Fos B localizes to the nucleus where it functions as a DNA-binding phosphoprotein (Keywords: Nucleus, DNA-binding, Phosphoprotein).

• Post-translational modifications include disulfide bond formation and phosphorylation, which may regulate its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human Fos B, which may allow recognition of both isoform 1 and isoform 2 if the epitope is conserved.

• For Western blot (WB), a band of approximately 36kDa is expected; consider using nuclear extracts or whole cell lysates from human samples.

• For immunohistochemistry (IHC-P), nuclear staining may be observed; antigen retrieval and antibody titration are recommended for optimal signal.

• The antibody is applicable to ELISA; validate the assay conditions in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw-material sourcing partner, CamelBio provides reliable access to this anti-Fos B rabbit monoclonal antibody and a comprehensive portfolio of antibodies for transcription factor and neurobiology research. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio streamlines procurement for diagnostic manufacturers, labs, and research institutes from concept to clinic.