Anti-FOPNL Polyclonal Antibody for WB, ELISA - Q96NB1

Core Product Specifications and Parameters

Biological Background: FOPNL (Centrosomal protein 20) Function and Localization

• FOPNL (FGFR1OP N-terminal-like protein), also known as Centrosomal protein 20, FOR20, C16orf63, and PHSECRG2, is a 20 kDa LisH domain-containing protein implicated in cilia biology.
• Plays a key role in the biogenesis of cilia. Related references: PMID:20551181
• Required for the recruitment of PLK1 to centrosomes and S phase progression. Related references: PMID:24018379
• Localizes to the centrosome, centriole, cilium, basal body, cytoplasmic granules, and centriolar satellites.
• Widely expressed in tissues including brain, heart, kidney, liver, lung, skeletal muscle, placenta, and intestine.
• Classified as a phosphoprotein and reference proteome member, with alternative splicing reported.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–100 of human FOPNL; therefore, this antibody may detect both full-length and N-terminal variants.
• For Western blot, expect a band near 20 kDa. Use human or mouse cell lysates (e.g., HEK293, HeLa) as positive controls.
• In ELISA, titrate the antibody and antigen to optimize signal; validate with recombinant FOPNL or appropriate cell extracts.
• As a polyclonal reagent, lot-to-lot consistency should be verified in the end-user's assay system.
• Cilia and centrosome structures may require specialized fixation or isolation protocols; validate in your specific sample preparation workflow.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this FOPNL polyclonal antibody as part of a comprehensive portfolio of IVD raw materials for centrosome and cilia research. Our one-stop sourcing model meets the needs of diagnostic manufacturers and labs with validated antibodies, bulk ancillary reagents, and custom development services, streamlining your transition from concept to clinic.