Anti-FOLR1 / Folate Binding Protein (FRalpha) Rabbit PolymAb® Monoclonal Antibody for WB, ELISA - P15328

Core Product Specifications and Parameters

Biological Background: Folate Receptor Alpha (FOLR1) Function and Localization

• FOLR1 binds folate and mediates cellular uptake of 5-methyltetrahydrofolate and folate analogs.
  Related references: PMID:19074442, PMID:23851396, PMID:23934049
• It exhibits high affinity for folate at neutral pH, which is critical for its transport function.
  Related references: PMID:23851396, PMID:23934049, PMID:2527252
• Endocytosis followed by acidic pH triggers a conformational change that releases bound folate.
  Related references: PMID:8567728
• The protein is anchored to the cell membrane via a GPI anchor and is also found in cytoplasmic vesicles and endosomes, reflecting its trafficking role.
• FOLR1 is primarily expressed in epithelial cells, with elevated expression in malignancies, and is also detected in kidney, lung, cerebellum, placenta, and thymus.
• Keywords include disulfide bond, glycoprotein, GPI-anchor, indicating post-translational modifications important for structure and function.
• The FOLR1 gene encodes a 29.8 kDa protein, which migrates at approximately 30 kDa on SDS-PAGE due to glycosylation.

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to the glycosylated protein is expected at approximately 30 kDa under reducing conditions.
• For ELISA, consider optimizing coating conditions and antibody concentrations using the target antigen or relevant biological samples.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for preclinical studies in these species; however, validation in the specific model is recommended.
• Optimal dilutions and incubation times should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, from lead identification to scaled-up production. This anti-FOLR1 monoclonal antibody serves as a robust tool for developing folate receptor-targeted diagnostics, and we additionally offer matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare-target raw materials to accelerate your assay development.