Anti-Follistatin (FST) Rabbit Polyclonal Antibody for WB, ELISA - P19883

Core Product Specifications and Parameters

Biological Background: Follistatin Function and Localization

• Follistatin is a secreted regulatory protein that binds and neutralizes TGF-beta superfamily ligands, including activin, myostatin, GDF11, and BMPs, by obstructing their type II receptor-binding site and inhibiting downstream signaling cascades. Related references: PMID:11279126, PMID:16482217, PMID:18535106
• As a specific inhibitor of pituitary follicle-stimulating hormone (FSH), follistatin sequesters activin A, reducing FSH biosynthesis and secretion. Related references: PMID:11279126
• It plays an essential role in muscle fiber formation and growth by counteracting myostatin and also through SMAD3/AKT/mTOR signaling independently of myostatin.
• Follistatin promotes neural differentiation by antagonizing BMP4 action.
• Interestingly, follistatin can translocate to the nucleus and nucleolus, where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis.
• Isoform 1 is the predominant circulating isoform, while embryonic expression is prominent in developing palatal epithelia and mesenchyme. Related references: PMID:31215115
• Post-translational modifications include disulfide bonds and glycosylation, with a predicted molecular weight of approximately 38 kDa.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide corresponding to residues 300-400 of human follistatin (P19883). For WB, consider using whole-cell lysates or tissue extracts from human or rat samples, as cross-reactivity is reported for these species. Optimize dilution factors empirically.
• For ELISA, validate using recombinant follistatin or relevant biological fluids. Since the immunogen is near the C-terminus, note that the antibody may detect all known isoforms if the epitope is conserved. Validate in the relevant sample system to confirm specificity.

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CamelBio provides diagnostic manufacturers and research institutes with streamlined access to a wide range of IVD raw materials, including validated antibodies like anti-follistatin polyclonal antibodies for reproductive biology and muscle research. Our one-stop platform also offers ancillary reagents, technical support, and customized sourcing for rare targets to accelerate your assay development from concept to clinic.