Anti-Folate Binding Protein (FBP) / FOLR1 Rabbit Monoclonal Antibody for WB - P15328

Core Product Specifications and Parameters

Biological Background: Folate Binding Protein (FBP)/FOLR1 (Folate receptor alpha) Function and Localization

• Folate receptor alpha (FOLR1) is a high-affinity folate binder that mediates cellular uptake of 5-methyltetrahydrofolate and folate analogs.
  Related references: PMID:19074442, PMID:23851396, PMID:23934049
• The protein exhibits pH-sensitive binding: high affinity at neutral pH, with endosomal acidification triggering a conformational change that releases folates.
  Related references: PMID:23851396, PMID:23934049, PMID:2527252, PMID:8567728
• FOLR1 is essential for normal embryonic development and cell proliferation (By similarity).
• Subcellular localization includes the cell membrane (apical and basolateral), cytoplasmic vesicles, clathrin-coated vesicles, endosomes, and secretion.
• Primarily expressed in epithelial tissues, with elevated expression in malignant tissues; detected in kidney, lung, cerebellum, placenta, and thymus epithelium.
• Classified as a GPI-anchored glycoprotein with disulfide bonds; involved in transport and linked to neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human FOLR1; consider this sequence when assessing isoform specificity or cross-reactivity.
• For Western blot, a band near 30 kDa may be observed; validate the signal in your target tissue or cell line.
• In IHC-P, expect enriched staining in epithelial-derived tissues and tumors; optimize antigen retrieval and antibody dilution for your sample type.
• For ELISA, validate the antibody pair using recombinant folate receptor alpha or cell lysates; check compatibility with your detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges diagnostic manufacturers with a comprehensive portfolio of IVD raw materials, including validated anti-FOLR1 monoclonal and polyclonal antibodies for folate metabolism and oncology applications. Beyond antibodies, our one-stop platform supplies bulk ancillary reagents and supports rare-target raw-material sourcing to accelerate your assay development from concept to clinic.