Anti-FNTB Monoclonal Antibody for WB, IHC-P, ELISA - P49356

Core Product Specifications and Parameters

Biological Background: FNTB Function and Localization

• FNTB encodes the beta subunit of protein farnesyltransferase, also known as CAAX farnesyltransferase subunit beta or Ras proteins prenyltransferase beta.
• It is the catalytic component that transfers a farnesyl moiety from farnesyl diphosphate to a cysteine residue in the C-terminal CAAX motif (Cys-aliphatic-aliphatic-X) of target proteins.
• This prenylation modifies small GTPases like Ras, regulating their membrane localization and activity.
• The enzyme complex requires zinc for activity (Metal-binding, Zinc).
• Post-translational modifications include phosphorylation (Phosphoprotein).
• Alternative splicing produces multiple isoforms (Alternative splicing).
• Structural studies have revealed the enzyme's 3D architecture (3D-structure).

Experimental Guidance and Technical Tips

• The immunogen (N-terminal peptide, aa 1-100 of human FNTB) may contribute to the antibody’s target specificity; verify in relevant model systems.
• For Western blot, the predicted band is ~49 kDa. Use freshly prepared lysates from human or rat cells and confirm band identity with knockdown/knockout controls if available.
• In immunohistochemistry (paraffin), antigen retrieval protocols may need optimization; test with known positive and negative tissues.
• ELISA applications can be developed using a direct or sandwich format; validate the antibody’s performance under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers with high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for prenylation research. Our streamlined supply chain covers rare target raw-material sourcing, enabling efficient assay development from concept to clinic.