Anti-FNTA Polyclonal Antibody for WB, ELISA - P49354

Core Product Specifications and Parameters

Biological Background: FNTA Function and Localization

• FNTA is the alpha subunit of both farnesyltransferase and geranylgeranyltransferase type-1 complexes, essential for post-translational lipid modification of proteins.
• It catalyzes the transfer of a farnesyl or geranylgeranyl group to the cysteine residue of proteins with a C-terminal CAAX motif (Cys-aliphatic-aliphatic-X).
• Through prenylation of RAC1, FNTA positively regulates neuromuscular junction development downstream of MUSK signaling.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, and requires magnesium as a cofactor.
• Alternative splicing of the FNTA gene generates multiple isoforms, which may have tissue-specific expression or functional differences.
• FNTA is a member of the protein prenyltransferase family and contains conserved repeat sequences important for its catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 280-379) of human FNTA, likely recognizing all isoforms; consider using peptide competition assays to confirm specificity.
• For Western blotting, the target protein migrates at ~44 kDa; validate in lysates from human, mouse, or rat samples and optimize primary antibody dilution carefully.
• In ELISA applications, the antibody may be suitable for both indirect and sandwich formats; pair with a validated detection antibody and determine optimal working concentrations experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a seamless sourcing bridge for IVD raw materials. With this anti-FNTA polyclonal antibody, we offer access to validated antibody reagents for prenylation studies, alongside a wide portfolio of monoclonal and polyclonal antibodies, bulk reagents, and rare target sourcing to accelerate your assay development.