Anti-FNDC5 Rabbit Polyclonal Antibody for WB, ELISA - Q8NAU1

Core Product Specifications and Parameters

Biological Background: FNDC5 Function and Localization

• FNDC5 (fibronectin type III domain-containing protein 5), also known as irisin and FRCP2, is a transmembrane protein that can be cleaved and secreted as the hormone irisin.
• The protein mediates the beneficial metabolic effects of muscular exercise by inducing browning of white adipose tissue, at least in part through stimulation of UCP1 expression via the nuclear receptor PPARA.
• FNDC5 localizes to the cell membrane and peroxisome membrane and is also secreted from the cell.
• It is widely expressed in human tissues, with highest levels detected in the heart, while expression is very low or absent in colon, pancreas, and spleen.
• The FNDC5 gene undergoes alternative splicing and alternative promoter usage, producing multiple isoforms.
• Keywords from UniProt indicate that FNDC5 is a glycoprotein, a hormone, and contains a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen was designed from a synthetic peptide within amino acids 113–212 of human FNDC5; consider sequence alignment if working with non-human species.
• For Western blot detection, validate in a relevant sample system—heart tissue or cell lines expressing FNDC5 may be appropriate given high cardiac expression.
• The antibody demonstrates cross-reactivity with rat; if using other species, confirm reactivity experimentally before large‑scale experiments.
• ELISA assays should be optimized with appropriate coating conditions and detection antibodies; verify linearity and specificity using recombinant protein or known positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare‑target raw materials. For FNDC5‑related research, we supply tools to support exercise metabolism and adipose biology studies, helping diagnostic manufacturers and laboratories accelerate development from concept to clinical application.