Anti-FNDC4 Polyclonal Antibody for WB, ELISA - Q9H6D8

Core Product Specifications and Parameters

Biological Background: FNDC4 Function and Localization

• FNDC4 (Fibronectin type III domain-containing protein 4), also known as fibronectin type III repeat-containing protein 1 (FRCP1), is a secreted and transmembrane glycoprotein.
• It acts as an anti-inflammatory factor, particularly in the colon where it down-regulates inflammation in macrophages.
• FNDC4 may suppress osteoclastogenesis and inhibit mature osteoclast resorptive function.
• In white adipose tissue, FNDC4 decreases local inflammation by interacting with GPR116, and it sensitizes white adipocytes to insulin, promoting glucose uptake, thereby improving systemic glucose tolerance.
• Subcellularly, FNDC4 localizes to the membrane and is also secreted, consistent with its roles in extracellular signaling.
• It is highly expressed in the liver and brain (cortex, hypothalamus, hippocampus), as well as in adipose tissue. Related references: PMID:34016966
• The protein is N-glycosylated and contains a signal peptide and a transmembrane helix, features typical of membrane and secreted proteins.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein spanning amino acids 45-167 of FNDC4, which covers part of the extracellular fibronectin type III domain; consider its specificity in the context of the target's membrane and secreted forms.
• For Western blot, the predicted molecular weight is approximately 25 kDa; use appropriate positive controls (e.g., human, mouse, or rat tissues expressing FNDC4) and validate in the relevant sample system.
• For ELISA, optimization of coating and detection conditions may be necessary to account for the secreted and membrane forms; consider using recombinant FNDC4 as a standard.
• When working with human, mouse, and rat samples, cross-reactivity is expected, but confirmatory validation in each species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with validated antibodies like this FNDC4 polyclonal antibody, essential for studying inflammation and metabolic disorders. We offer a wide range of bulk ancillary reagents, optimized polyclonal and monoclonal antibodies, and can assist in sourcing rare target raw materials. Our one-stop supply model supports your entire workflow from discovery to clinical application, ensuring you have the reliable tools needed for accurate and reproducible results.