Anti-FN3KRP Rabbit Polyclonal Antibody for WB, ELISA - Q9HA64

Core Product Specifications and Parameters

Biological Background: FN3KRP Function and Localization

• FN3KRP (Ketosamine-3-kinase), also known as FN3K-RP or fructosamine-3-kinase-related protein, is a transferase enzyme belonging to the kinase family.
• It catalyzes the phosphorylation of ribuloselysine and psicoselysine residues on glycated proteins, generating unstable adducts that decompose under physiological conditions, thus mediating protein deglycation. Related references: PMID:14633848 PMID:15137908
• Unlike the related kinase FN3K, FN3KRP does not phosphorylate fructoselysine, demonstrating distinct substrate specificity.
• The protein is widely expressed in human tissues, with very low levels observed in skeletal muscle; it is also present in erythrocytes. Related references: PMID:15331600 PMID:15137908
• According to UniProt annotation, FN3KRP contains ATP-binding and nucleotide-binding regions and is characterized as a phosphoprotein, consistent with its kinase function.
• Its calculated molecular weight is approximately 34 kDa, as reported in UniProt (34412 Da).

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human FN3KRP (residues 1–309), which may enable recognition of multiple epitopes and robust detection in denaturing Western blot assays.
• For ELISA applications, consider testing the antibody in both direct and indirect formats, especially when the target is expected in its native state.
• Validate detection in relevant biological samples (e.g., erythrocytes, known to express FN3KRP) and across the indicated cross-reactive species (human, mouse, rat).
• In Western blotting, expect a band near 34 kDa; use appropriate molecular weight markers and optimize loading controls for your sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a seamless, one‑stop sourcing platform for IVD raw materials, from concept to clinic. For studies focused on protein deglycation and kinase biology, we supply validated antibodies like anti-FN3KRP, along with antibody pairs, bulk ancillary reagents, and rare‑target sourcing support. Our integrated services help streamline your development pipeline with reliable, research‑grade materials and technical consultation.