Anti-FN1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P02751

Core Product Specifications and Parameters

Biological Background: Fibronectin Function and Localization

• Fibronectin is a high-molecular-weight glycoprotein that binds to cell surfaces and various extracellular matrix components, including collagen, fibrin, heparin, DNA, and actin. It is essential for cell adhesion, migration, opsonization, wound healing, and the maintenance of cell morphology. Related references: PMID:3024962, PMID:3593230, PMID:3900070
• Fibronectin acts as a ligand for the LILRB4 receptor, thereby inhibiting FCGR1A/CD64-mediated monocyte activation. Related references: PMID:34089617
• It is involved in osteoblast compaction and regulation of type I collagen deposition, critical for bone mineralization (By similarity).
• The protein exists as multiple isoforms due to alternative splicing: plasma fibronectin (soluble dimer) secreted by hepatocytes, and cellular fibronectin (dimeric or multimeric) deposited as fibrils by fibroblasts and other cell types. Tissue-specific expression includes retina and urine.
• Subcellular localization: Secreted into the extracellular space and extracellular matrix.
• An internal fragment, anastellin, binds fibronectin to induce superfibronectin formation with enhanced adhesive properties, and inhibits tumor growth, angiogenesis, and metastasis.
• Fibronectin secreted by contracting muscle promotes liver autophagy and systemic insulin sensitization through ITGA5:ITGB1 integrin signaling.
• Keywords: Acute phase, Alternative splicing, Angiogenesis, Cell adhesion, Disulfide bond, Glycoprotein, Heparin-binding, Oxidation, Phosphoprotein, Repeat, Secreted, Signal, Sulfation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 2205–2477 in the C-terminal region of human fibronectin (isoform 1), encompassing the heparin-binding domain and part of the fibrin-binding region. This design may favor detection of full-length fibronectin and certain splice variants containing this segment.
• For Western blotting, multiple bands may be observed at approximately 72 kDa, 74 kDa, and 221–272 kDa, likely reflecting proteolytic fragments and full-length or multimeric forms. Optimize sample preparation and reducing conditions to minimize degradation.
• In immunohistochemistry and immunofluorescence, consider antigen retrieval methods and fixation conditions; fibronectin is an abundant ECM protein, so background may require blocking with serum or BSA.
• For ELISA and immunoprecipitation, validate the antibody with recombinant fibronectin standards or known positive controls to confirm sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw material sourcing partner, providing monoclonal and polyclonal antibodies, validated antibody pairs, and ancillary reagents for diagnostic development. The Anti-FN1 Rabbit Monoclonal Antibody (SWISS P02751) supports research in extracellular matrix biology, cell adhesion, and wound healing. Leverage CamelBio's expertise to streamline your procurement of rare-target reagents and bulk raw materials.