Anti-FMRP Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q06787

Core Product Specifications and Parameters

Biological Background: FMRP Function and Localization

• FMRP is a multifunctional polyribosome-associated RNA-binding protein essential for neuronal development and synaptic plasticity. It regulates alternative mRNA splicing, mRNA stability, dendritic mRNA transport, and postsynaptic local protein synthesis. Related references: PMID:12417522, PMID:16631377, PMID:18653529
• Acts as a repressor of mRNA translation, particularly at synapses, by forming neuronal ribonucleoprotein granules and recruiting EIF4EBP2; it undergoes liquid-liquid phase separation upon target mRNA binding and phosphorylation. Related references: PMID:30765518, PMID:31439799
• Binds to G-quadruplex structures and specific RNA motifs such as 5'-ACU[GU]-3' and 5'-[AU]GGA-3' in target mRNAs, mediating translation regulation and mRNA localization. Related references: PMID:11719189, PMID:23235829, PMID:25464849
• Localizes to multiple subcellular compartments: cytoplasm, stress granules, neuronal projections (axons, dendrites, dendritic spines), synapses, nucleus, and Cajal bodies, reflecting its roles in mRNA transport, translation, and nuclear export.
• Implicated in mRNA nuclear export by specifically recognizing N6-methyladenosine (m6A)-containing mRNAs and promoting their export in a CRM1-dependent manner. Related references: PMID:31753916
• Highly expressed in brain, cerebellum, testis, and also found in epithelial tissues, neurons, Purkinje cells, spermatogonia, oligodendrocytes, and lymphocytes. Related references: PMID:8401578, PMID:9259278, PMID:23891804, PMID:8504300
• Post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination regulate FMRP activity, phase separation, and protein interactions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 500–600 of human FMRP; the antibody may recognize full-length FMRP and any isoforms containing this region. Validate specificity in your experimental system.
• For western blot (WB), a band near 71 kDa is expected, though post-translational modifications or isoform variations may shift migration. Include appropriate controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider using tissue or cell types with high FMRP expression (e.g., brain, testis) and optimize fixation/permeabilization conditions.
• For ELISA, peptide-based detection may be optimized; consider pairing with a validated capture/detection system.
• Cross-reactivity is reported for human, mouse, and rat. Validate in the relevant species, as sequence homology in the immunogen region may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a seamless sourcing experience for IVD raw materials, technical services, and consulting—from concept to clinic. For FMRP-targeted research and assay development, our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and access to rare target raw materials. Partner with CamelBio to streamline procurement and accelerate your translational projects in neuronal biology and diagnostics.