Anti-FMRP Monoclonal Antibody for WB, IHC-P, ELISA - Q06787

Core Product Specifications and Parameters

Biological Background: FMRP Function and Localization

• FMRP (Fragile X messenger ribonucleoprotein 1), encoded by the FMR1 gene, is a multifunctional polyribosome-associated RNA-binding protein crucial for neuronal development and synaptic plasticity. It regulates alternative mRNA splicing, mRNA stability, dendritic transport, and local protein synthesis at synapses. Related references: PMID:12417522 PMID:16631377 PMID:18653529 PMID:30765518 PMID:31439799 PMID:11157796 PMID:11532944 PMID:12594214
• Acts as both a translational repressor and activator of target mRNAs. In synapses, it typically represses translation via the CYFIP1-EIF4E-FMR1 complex and by forming phase-separated ribonucleoprotein granules that inhibit translation. However, upon metabotropic glutamate receptor (mGluR) stimulation, it can activate translation of a subset of dendritic mRNAs. Related references: PMID:12417522 PMID:31439799 PMID:30765518 PMID:11157796 PMID:11532944 PMID:12594214
• Binds to specific RNA motifs including G-quadruplex structures, U-rich sequences, and the ACU[GU] and [AU]GGA consensus sequences, primarily within coding sequences and 3'UTRs of target mRNAs. It also interacts with the non-coding BC1 RNA and various miRNAs. Related references: PMID:23235829 PMID:11719189 PMID:18579868 PMID:25464849 PMID:12927206
• Localizes extensively in the cytoplasm, particularly at neuronal projections including dendrites, axons, dendritic spines, and growth cones. It is found in ribonucleoprotein granules, stress granules, and at synaptic sites (both pre- and postsynaptic). Nuclear localization includes the nucleolus, Cajal bodies, and centromere. It also associates with the cell membrane.
• Post-translationally modified by acetylation, methylation, and phosphorylation (including phase-separation-promoting phosphorylation). Ubiquitination is also noted. Mutations in FMR1 cause fragile X syndrome (intellectual disability) and premature ovarian failure; the protein is implicated in neurodegeneration.
• Exhibits tissue-specific expression, mainly in brain, cerebellum, testis, and epithelial tissues. At the protein level, detected in neurons, Purkinje cells, spermatogonias, and lymphocytes.
• In vitro, FMRP binds preferentially to poly(G) and poly(U) RNA homopolymers. Related references: PMID:12950170 PMID:15381419 PMID:7688265 PMID:24204304

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering residues 1–100 of human FMRP (Q06787); thus, the antibody is expected to recognize the N-terminal region. For western blot (WB), a band at ~71 kDa is predicted; consider using lysates from brain or testis (high endogenous expression) as positive controls.
• For immunohistochemistry (IHC-P), optimal staining may require antigen retrieval and careful titration; validation in human, mouse, or rat neuronal tissues is recommended.
• In ELISA, the peptide immunogen can serve as a standard for quantitative assays; paired with an appropriate detection antibody, this clone may be suitable for sandwich ELISA development.
• As a rabbit monoclonal antibody, this reagent provides consistent lot-to-lot reproducibility and high affinity, but each new lot should be validated in the end-user’s specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, from validated antibodies and antigens to technical consultation. This anti-FMRP antibody exemplifies our commitment to providing high-quality reagents for neuroscience and genetic disease research. Whether you need pre-qualified antibody pairs, custom monoclonal development, or bulk ancillary reagents, CamelBio serves as your integrated procurement partner, helping streamline your development from concept to clinical application.