Anti-FMO3 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P31513

Core Product Specifications and Parameters

Biological Background: FMO3 Function and Localization

• FMO3 is a flavin-containing monooxygenase (FMO) that catalyzes the NADPH-dependent oxygenation of various nitrogen- and sulfur-containing compounds, including drugs and dietary components. Related references: PMID:10759686, PMID:30381441, PMID:32156684
• It plays a critical role in the metabolism of trimethylamine (TMA) to trimethylamine N-oxide (TMAO), a process that influences platelet responsiveness and thrombus formation. Related references: PMID:9776311, PMID:29981269
• The enzyme is localized to the endoplasmic reticulum (microsomal) membrane.
• FMO3 is predominantly expressed in the liver.
• It contains FAD and NADP binding sites and is classified as a monooxygenase.
• Post-translational modifications include phosphorylation (phosphoprotein).
• The protein has a molecular weight of approximately 60 kDa.

Experimental Guidance and Technical Tips

• For Western blot, expect a band near 60 kDa in human liver lysates; consider using a positive control tissue or cell line expressing FMO3.
• In immunofluorescence (IF-P), optimize permeabilization conditions due to the ER membrane localization of FMO3.
• As a monoclonal antibody, it offers consistent batch-to-batch performance; validate in the relevant sample system for your assay.
• Cross-reactivity with mouse and rat suggests a conserved epitope; validate detection sensitivity in these species if used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials and technical support, from concept to clinic. This anti-FMO3 rabbit monoclonal antibody supports research in hepatic drug metabolism and trimethylamine N-oxide biology. Beyond this product, we offer validated antibody pairs, bulk reagents, and customized sourcing for rare targets to streamline your assay development.