Anti-FMO1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q01740

Core Product Specifications and Parameters

Biological Background: FMO1 Function and Localization

• FMO1 (flavin-containing monooxygenase 1) is a broad-spectrum monooxygenase that catalyzes the oxygenation of numerous nitrogen- and sulfur-containing compounds, including xenobiotics and endogenous substrates like hypotaurine.
• It converts hypotaurine to taurine via S-oxygenation, an important osmolyte involved in cell volume regulation and cytoprotection. Related references: PMID:32156684
• The enzyme also N-oxygenates trimethylamine (TMA) to trimethylamine N-oxide (TMAO) in vitro, potentially contributing to detoxification of gut microbiota-derived metabolites.
• FMO1 is a member of the flavin-containing monooxygenase family, characterized by FAD and NADP cofactor binding.
• Localized to the endoplasmic reticulum membrane, consistent with its role in cellular detoxification pathways.
• Expressed primarily in fetal and adult human liver, suggesting a specialized role in hepatic metabolism.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 371-470 of human FMO1; consider using peptide blocking to confirm specificity.
• For western blotting, a band at approximately 60 kDa is expected; validate detection in human liver lysates or FMO1-overexpressing cell lines.
• In immunofluorescence, anticipate ER-localized staining; counterstain with ER markers for co-localization studies.
• The antibody shows cross-reactivity with rat FMO1; verify performance in the relevant species before quantitative assays.
• ELISA applications may benefit from using recombinant FMO1 as a standard to establish detection range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials tailored for drug metabolism and detoxification research, including validated polyclonal antibodies like this anti-FMO1 reagent. We offer reliable sourcing of optimized antibodies, bulk ancillary reagents, and customized raw-material solutions to streamline your diagnostic development from concept to clinic. Partner with us to secure high-quality, cost-effective supplies for your metabolic and toxicological assay needs.