Anti-FLT3 Polyclonal Antibody for WB, IHC-P, ELISA - P36888

Core Product Specifications and Parameters

Biological Background: FLT3 Function and Localization

• FLT3 (also known as CD135, FLK2, STK1, FLK-2) is a receptor-type tyrosine-protein kinase that serves as the cell-surface receptor for the cytokine FLT3LG.
• It plays a critical role in regulating differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells.
• Upon ligand binding, FLT3 activates multiple downstream signaling pathways, including RAS/MAPK, PI3K/AKT/mTOR, and STAT5, by phosphorylating key mediators such as SHC1, AKT1, and PLCG1.
• FLT3 is predominantly expressed in bone marrow, hematopoietic stem cells, myeloid progenitor cells, and granulocyte/macrophage progenitor cells, with lower levels in kidney, pancreas, and high expression in T-cell leukemia.
• It is localized to the cell membrane and endoplasmic reticulum lumen, consistent with its role as a transmembrane receptor.
• Keywords highlight its involvement as a proto-oncogene, with disease-associated mutations causing constitutive kinase activity linked to leukemia.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination, which regulate its stability and signaling.
• Alternative splicing produces multiple isoforms, potentially affecting ligand binding and kinase activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the intracellular region (amino acids 701-800) of human FLT3, which includes part of the tyrosine kinase domain. This region is highly conserved, suggesting potential cross-reactivity with mouse and rat FLT3 as indicated by the supplier.
• For Western blotting, a band at approximately 113 kDa is expected. Optimize blocking and antibody dilution using appropriate positive control lysates (e.g., hematopoietic cell lines).
• For immunohistochemistry (IHC-P), antigen retrieval and detection conditions should be validated using tissue sections known to express FLT3, such as bone marrow or leukemia samples.
• For ELISA, the use of peptide immunogen may enhance sensitivity; consider pairing with a detection antibody against a distinct epitope for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with integrated access to IVD raw materials, including validated antibodies like this anti-FLT3 polyclonal antibody. For hematopoietic malignancy and oncology research applications, we offer one-stop sourcing of matched antibody pairs, bulk reagents, and customized development services to streamline your assay development.