Anti-Flotillin 1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75955

Core Product Specifications and Parameters

Biological Background: Flotillin 1 Function and Localization

• Flotillin-1 is a protein encoded by the FLOT1 gene, known for its role as a scaffolding protein within caveolar membranes.
• It may participate in the formation of caveolae or caveolae-like vesicles, contributing to membrane organization and endocytosis.
• Flotillin-1 contains a SPFH (stomatin, prohibitin, flotillin, and HflK/C) domain, characteristic of lipid raft-associated proteins.
• Subcellular localization includes the cell membrane, endosomes, melanosomes, and detergent-resistant membrane rafts.
• Alternative splicing and phosphorylation are documented regulatory mechanisms, as indicated by UniProt keywords.
• The protein has a predicted molecular weight of approximately 47 kDa, consistent with the observed band in Western blot.
• It is ubiquitously expressed, with higher levels in brain, heart, lung, and placenta (inferred from general flotillin knowledge).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 128–427, covering a large portion of the protein; the resulting polyclonal antibody may detect multiple isoforms or degradation fragments.
• For Western blot, a band near 47 kDa is expected; consider using reducing and non-reducing conditions to assess sensitivity.
• IHC-P may benefit from antigen retrieval steps (e.g., citrate buffer pH 6.0) and careful optimization of fixation methods.
• Cross-reactivity with mouse and rat is indicated, but target sequence homology should be verified in your specific experimental system.
• For ELISA, the antibody can be used as a capture or detection reagent, but pairing with a suitable monoclonal may improve specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to core raw materials for membrane and cell biology studies. This Flotillin 1 antibody is part of our curated portfolio supporting caveolin, lipid raft, and endocytosis research. Whether you need validated antibody pairs, recombinant proteins, or bulk ancillary reagents, our one-stop sourcing model helps accelerate your projects from concept to clinic.