Anti-Flotillin 1 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - O75955

Core Product Specifications and Parameters

Biological Background: Flotillin-1 Function and Localization

• Flotillin-1 (FLOT1) is a highly conserved protein and a member of the flotillin/reggie family; alternative splicing generates multiple isoforms.
• It acts as a scaffolding protein within caveolar membranes and lipid rafts, functionally contributing to the formation of caveolae or caveolae-like vesicles.
• Flotillin-1 localizes to the cell membrane, specifically in caveolae and membrane rafts, and is also found in endosomes and melanosomes.
• The protein is subject to post-translational modifications, including phosphorylation, which may regulate its function and interactions.
• Structural studies have provided insights into its oligomerization and membrane-binding, as indicated by the '3D-structure' keyword in UniProt.
• It plays a role in endocytosis, signal transduction, and actin cytoskeleton reorganization, partly due to its association with membrane microdomains.
• Flotillin-1 is widely expressed but shows enrichment in tissues with abundant caveolae, such as adipose tissue and lung.
• As a proteomics identification standard, Flotillin-1 is frequently used as a marker for lipid raft and caveolae purification.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 155–427, covering a significant portion of the C-terminal domain; the antibody may detect multiple isoforms or cleavage products—validate the observed band pattern in your sample system.
• For Western blotting, expect a main band at approximately 47 kDa; consider using reducing conditions and confirm specificity with knockout controls or siRNA knockdown where available.
• In immunofluorescence and IHC, Flotillin-1 typically displays plasma membrane and punctate cytoplasmic staining; include membrane markers and perform proper fixation/permeabilization to preserve epitopes.
• For immunoprecipitation, the antibody is listed as IP-capable; optimize the amount of antibody and use appropriate detergent conditions to maintain protein interactions, as Flotillin-1 is tightly associated with lipid rafts.
• Cross-reactivity is noted for human, mouse, and rat; if using other species, verify sequence homology of the immunogen region and perform a pilot test.
• Always titrate the antibody for your specific application and include relevant positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio specializes in providing diagnostic manufacturers and research laboratories with a comprehensive selection of IVD raw materials. This anti-Flotillin-1 polyclonal antibody is well-suited for caveolae and membrane raft research, and we offer a full range of ancillary reagents.
• Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk quantities of ancillary reagents, and assist in sourcing raw materials for rare targets—supporting your projects from concept to clinic.