Anti-Flotillin-1 Monoclonal Antibody for WB, IHC-P, ELISA - O75955

Core Product Specifications and Parameters

Biological Background: Flotillin-1 Function and Localization

• Flotillin-1 (FLOT1) is a 47 kDa protein encoded by the FLOT1 gene in humans and belongs to the flotillin family of lipid raft-associated proteins.
• It functions as a scaffolding protein within caveolar membranes and may participate in the formation of caveolae or caveolae-like vesicles, contributing to membrane curvature and vesicle trafficking.
• The protein localizes predominantly to the cell membrane, particularly in caveolae and membrane rafts, but is also found in endosomes and melanosomes, indicating roles in endocytosis and organelle biogenesis.
• Flotillin-1 undergoes alternative splicing, yielding isoforms that may have distinct functions or localizations.
• It is a phosphoprotein, suggesting regulation by kinase signaling pathways, and has a known 3D structure, facilitating structure-function studies.
• It is widely used as a marker for lipid rafts and is part of the reference proteome, underscoring its fundamental role in cell biology.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a recombinant protein spanning amino acids 155–427 of human Flotillin-1, which covers the C-terminal region containing conserved flotillin domains. It is likely to recognize full-length endogenous Flotillin-1 in human, mouse, and rat samples.
• For WB, a band at ~47 kDa is expected. Use standard reducing conditions and whole-cell lysates from cell lines such as HeLa or HEK293. KO validation underscores its specificity; consider using knockout cells as a negative control.
• For IHC-P, test on formalin-fixed paraffin-embedded tissue sections with heat-induced epitope retrieval. Staining may be membranous and/or punctate, reflecting caveolar/endosomal localization.
• For ELISA, employ as a capture or detection antibody in sandwich immunoassays after confirming pairing compatibility. Validate assay conditions with recombinant Flotillin-1 protein.
• Note that the immunogen is a recombinant fusion protein; batch-to-batch consistency may affect performance. Optimize dilutions empirically in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, offering a broad portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-Flotillin-1 antibody serves research in membrane biology, signal transduction, and cancer diagnostics, and CamelBio’s integrated supply chain can support your scale-up from concept to clinic. Reach out to explore custom antibody development or procurement solutions tailored to your diagnostic pipeline.