Anti-Flotillin 1 Monoclonal Antibody for WB, IHC-P, ELISA - O75955

Core Product Specifications and Parameters

Biological Background: Flotillin 1 Function and Localization

• Flotillin-1 (FLOT1) is a scaffolding protein that participates in the formation of caveolae or caveolae-like vesicles at the plasma membrane.
• It localizes to the cell membrane, specifically in caveolae, membrane rafts, and also in endosomes and melanosomes.
• FLOT1 undergoes alternative splicing and is classified as a phosphoprotein, indicating potential regulation by post-translational modifications.
• The human FLOT1 gene (GeneID: 10211) encodes a protein with a calculated molecular weight of approximately 47 kDa (UniProt: 47355 Da).
• UniProt keywords highlight its structural characterization (3D-structure) and identification through proteomics.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 328–427 of human FLOT1 (O75955). This C-terminal region may provide good specificity; nonetheless, confirm reactivity in your target species and sample type.
• For Western blotting, a band near 47 kDa is expected. Use appropriate loading controls and molecular weight markers.
• In immunohistochemistry (paraffin sections), antigen retrieval conditions may need optimization; validate with known positive (e.g., caveolin-rich tissues) and negative controls.
• Cross-reactivity with mouse and rat FLOT1 is indicated; check sequence alignment of the immunogen region for additional species if needed.
• ELISA applications can be assessed using recombinant FLOT1 protein or cell lysates, with attention to coating buffer and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD manufacturers, offering one-stop access to raw materials and technical services. For studies involving membrane organization and caveolae biology, CamelBio supplies validated anti-Flotillin 1 antibodies and related reagents to accelerate your assay development. From concept to clinic, rely on CamelBio for bulk ancillary materials, antibody pairs, and sourcing expertise.