Anti-FLI1 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q01543

Core Product Specifications and Parameters

Biological Background: FLI1 Function and Localization

• FLI1 (Friend leukemia integration 1 transcription factor) is a member of the ETS transcription factor family, also known as ERGB and proto-oncogene Fli-1.
• It functions as a sequence-specific transcriptional activator that recognizes the DNA motif 5'-C[CA]GGAAGT-3'. Related references: PMID:24100448 PMID:26316623 PMID:28255014
• FLI1 is predominantly localized in the nucleus, consistent with its role in transcription regulation.
• Chromosomal rearrangements involving FLI1 are implicated in Ewing sarcoma and other cancers.
• Alternative splicing generates multiple isoforms of the protein.
• Phosphorylation represents a key post-translational modification that may regulate its activity.
• Disease variants have been reported, and FLI1 serves as a proteomics identification marker.

Experimental Guidance and Technical Tips

• For Western blot, consider using this antibody to detect FLI1 at approximately 51 kDa in human and mouse cell lysates. The polyclonal nature may produce additional bands; validate specificity with appropriate controls.
• In immunoprecipitation, the antibody's binding to the N-terminal region (aa 1-100) of FLI1 may be useful for capturing full-length endogenous protein.
• For ELISA applications, optimize coating conditions and confirm reactivity with the recombinant immunogen.
• Cross-reactivity with mouse FLI1 suggests potential utility in rodent model systems, but validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-FLI1 pAb, bulk ancillary reagents, and rare target raw-material sourcing to support your transcription factor and oncology research programs.