Anti-FLG Rabbit Polyclonal Antibody for WB, ELISA - P20930

Core Product Specifications and Parameters

Biological Background: Filaggrin Function and Localization

• Filaggrin (FLG) is a large, histidine-rich, basic protein that aggregates keratin intermediate filaments in the epidermis, forming the structural matrix of the cornified cell envelope.
• It promotes disulfide-bond formation among intermediate filaments, contributing to the mechanical strength and barrier function of the skin.
• FLG is expressed predominantly in the stratum granulosum of the epidermis, where it is synthesized as a giant precursor, profilaggrin, which is subsequently proteolytically processed into active filaggrin monomers.
• Subcellularly, filaggrin localizes to cytoplasmic keratohyalin granules in granular layer keratinocytes.
• The protein contains multiple filaggrin repeats that facilitate keratin binding and filament aggregation, and its function is regulated by calcium ions and post-translational modifications like citrullination.
• As a developmental protein, FLG plays a critical role in the terminal differentiation of keratinocytes and the formation of a waterproof skin barrier.
• Mutations in the FLG gene are a major predisposing factor for ichthyosis vulgaris and atopic dermatitis, highlighting its clinical significance in skin disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-92 of human FLG (N-terminal region). This region may be conserved across species, consistent with reported cross-reactivity with mouse and rat. Validate cross-reactivity in your sample system if using non-human models.
• Recommended applications include Western blotting (WB) and ELISA. For WB, note the large molecular weight (~435 kDa); consider using a low-percentage gel and extended transfer times to ensure efficient protein transfer.
• Due to the high molecular weight and repetitive nature of filaggrin, consider using fresh or well-preserved samples to avoid protein degradation, and include protease inhibitors during sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-FLG antibody supports research in epidermal differentiation, skin barrier function, and dermatological diseases. For specialty targets like filaggrin, we provide custom antibody development and rare target raw-material sourcing to accelerate your assay development from concept to clinic.