Anti-FLAP Rabbit Monoclonal Antibody for WB, ELISA - P20292

Core Product Specifications and Parameters

Biological Background: Arachidonate 5-lipoxygenase-activating protein (ALOX5AP/FLAP) Function and Localization

• Arachidonate 5-lipoxygenase-activating protein (also known as FLAP or MK-886-binding protein) is encoded by the ALOX5AP gene in humans and is a critical component of the leukotriene biosynthesis pathway.
• FLAP anchors 5-lipoxygenase (ALOX5) to the nuclear and endoplasmic reticulum membranes, enabling the efficient transfer of arachidonic acid to ALOX5 for leukotriene production.
• Leukotrienes are potent lipid mediators involved in inflammatory and allergic responses, making FLAP a central regulator of inflammation and asthma pathogenesis.
• FLAP binds specifically to the inhibitory compound MK-886, which blocks leukotriene biosynthesis and validates FLAP as a pharmacological target for anti-inflammatory therapies.
• At the subcellular level, FLAP localizes predominantly to the nucleus membrane and endoplasmic reticulum membrane, consistent with its role in compartmentalizing arachidonic acid metabolism.
• Structurally, FLAP is a transmembrane protein with a molecular weight of approximately 18 kDa and contains multiple transmembrane helices, as highlighted by UniProt keywords.
• Additional annotations from curated databases include involvement in 3D-structure determination, proteomics identification, and its classification as a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 62–161) of human FLAP; this sequence may be conserved across species, but the product’s documented reactivity is limited to human.
• For Western blotting, the predicted molecular weight is 18 kDa; consider using 12–15% SDS-PAGE gels and optimized transfer conditions for low-molecular-weight proteins.
• Membrane protein extraction may require detergents (e.g., RIPA buffer) to ensure complete solubilization; include appropriate positive and negative controls.
• In ELISA applications, validate antibody performance using the immunizing peptide or recombinant FLAP protein as a standard.
• Given FLAP’s role in leukotriene biosynthesis, consider testing the antibody in leukocyte or airway epithelial cell models relevant to inflammation research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop platform for IVD raw material procurement, supporting diagnostic manufacturers from concept to clinic. For researchers investigating leukotriene biosynthesis and inflammatory pathways, we supply high-quality antibodies such as this FLAP rabbit monoclonal antibody, along with validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare or specialized targets. Our commitment is to streamline your supply chain so you can focus on accelerating assay development.