Anti-FKBP52/FKBP4 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q02790

Core Product Specifications and Parameters

Biological Background: FKBP52/FKBP4 Function and Localization

• FKBP52/FKBP4, also known as FKBP51, Hsp56, p59, or HBI, is a member of the immunophilin family with peptidyl-prolyl cis-trans isomerase (PPIase) and co-chaperone activities.
• It serves as a component of steroid receptor heterocomplexes by interacting with heat-shock protein 90 (HSP90), facilitating intracellular trafficking of steroid hormone receptors between the cytoplasm and nucleus.
• The protein is widely expressed and localizes to multiple cellular compartments, including the cytoplasm, cytosol, mitochondrion, nucleus, cytoskeleton, and neuronal axons.
• FKBP52/FKBP4 regulates neuronal growth cone dynamics through its isomerase activity by modulating TRPC1 channel opening, and also controls microtubule assembly by inhibiting MAPT/TAU.
• It may have a protective role against oxidative stress in mitochondria.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitin-like conjugation, as indicated by proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-92 of human FKBP4 (N-terminal region). This rabbit monoclonal antibody is expected to recognize the endogenous protein in human, mouse, and rat samples.
• For western blotting (WB), a band around 52 kDa is anticipated. Validate specificity in knockout or knockdown models and use appropriate loading controls.
• In immunofluorescence (IF/ICC) applications, consider the multilocalized nature of FKBP52/FKBP4 (cytoplasm, nucleus, mitochondria). Use organelle markers to confirm subcellular distribution.
• For ELISA, optimizing coating antigen and antibody dilution will help achieve robust signal-to-noise ratios.
• Cross-reactivity with mouse and rat has been indicated; however, experimental validation in the relevant species and tissue types is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers investigating immunophilin biology and steroid receptor signaling, we provide reliable access to FKBP52/FKBP4 antibodies and related co-chaperone targets. Our supply capabilities extend to rare-target raw-material sourcing and technical support, bridging the gap from concept to clinic.