Anti-FKBP52/FKBP4 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q02790

Core Product Specifications and Parameters

Biological Background: FKBP52/FKBP4 Function and Localization

• FKBP4 encodes the peptidyl-prolyl cis-trans isomerase FKBP4, commonly known as FKBP52, FKBP59, HBI, and p52, and is a member of the immunophilin family.
• Acts as an immunophilin with peptidyl-prolyl cis-trans isomerase (PPIase) and co-chaperone activities, facilitating protein folding and complex assembly.
• Serves as a component of steroid receptor heterocomplexes by interacting with heat-shock protein 90 (HSP90), playing a role in intracellular trafficking of hormone receptors between cytoplasm and nucleus.
• Regulates neuronal growth cone dynamics by modulating TRPC1 channel opening via its isomerase activity.
• Functions as a regulator of microtubule dynamics by inhibiting MAPT/TAU's ability to promote microtubule assembly.
• May confer a protective role against oxidative stress within mitochondria.
• Localizes to multiple subcellular compartments including cytoplasm, cytosol, mitochondria, nucleus, cytoskeleton, and axon projections.
• Widely expressed across various tissues, and its post-translational modifications include phosphorylation, acetylation, and methylation; structural features include TPR repeat domains.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 220‑459) targets a central region of FKBP4, so the antibody may recognize full‑length FKBP4 and potentially related isoforms if present; verify specificity in your model system.
• For Western blot (WB), use a predicted molecular weight of approximately 52 kDa; select a suitable blocking and detection protocol based on rabbit polyclonal antibody properties.
• In immunohistochemistry (IHC‑P), consider antigen retrieval conditions and validate staining in known positive tissues (e.g., human, mouse, rat samples) with appropriate controls.
• When performing immunoprecipitation (IP) or ELISA, include matched negative controls and confirm recovery of the target protein; cross‑reactivity data support detection across human, mouse, and rat species.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw‑material supplier, CamelBio streamlines procurement for diagnostic developers and researchers working on immunophilin, chaperone, and steroid‑receptor biology. We offer validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and custom sourcing for rare targets—all tailored to support your FKBP52/FKBP4 studies from concept to clinic. Rely on CamelBio as your single integrated partner for high‑quality reagents and technical support.