Anti-FKBP51/FKBP5 Polyclonal Antibody for WB, ELISA - Q13451

Core Product Specifications and Parameters

Biological Background: FKBP51/FKBP5 Function and Localization

• FKBP5 encodes the 51 kDa FK506-binding protein (FKBP51), also known as FKBP54, a member of the immunophilin family with peptidyl-prolyl cis-trans isomerase (PPIase) and co-chaperone activities. Related references: PMID:11350175
• It acts as a co-chaperone for heat-shock protein 90 (HSP90), forming part of unligated steroid hormone receptor complexes and regulating their intracellular trafficking and cytoplasmic retention (PubMed:12538866). Related references: PMID:12538866
• FKBP51 regulates Akt/AKT1 signaling by promoting interaction with PHLPP1, leading to dephosphorylation and activation of Akt (PubMed:28147277, PubMed:28363942). Related references: PMID:28147277 PMID:28363942
• It participates in innate immune signaling by interacting with IKBKE and IKBKB, facilitating IKK complex assembly, NF-kappa-B activation, and interferon production (PubMed:26101251, PubMed:31434731). Related references: PMID:26101251 PMID:31434731
• The protein localizes to both the cytoplasm and nucleus, and is widely expressed with enrichment in testis.
• Alternative splicing generates multiple isoforms, and post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 308-457) containing TPR repeat domains critical for HSP90 interaction; the antibody may recognize multiple isoforms if this region is conserved.
• For Western blot, expect a band around 51 kDa; include appropriate positive and negative controls to confirm specificity.
• The supplier reports cross-reactivity with mouse and rat samples; validate the antibody in your specific sample system before routine use.
• For ELISA, optimize coating and detection conditions as recommended for polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, offering diagnostic manufacturers and research labs validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. This FKBP51 antibody supports research into steroid receptor signaling, Akt pathway regulation, and immunophilin biology. Let CamelBio help streamline your procurement and accelerate your assay development from concept to clinic.