Anti-FKBP38/FKBP8 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q14318

Core Product Specifications and Parameters

Biological Background: FKBP38/FKBP8 Function and Localization

• FKBP38, also known as FKBP8, is a 38 kDa FK506-binding protein and peptidyl-prolyl cis-trans isomerase (PPiase) that belongs to the immunophilin family.
• It acts as a constitutively inactive PPiase that becomes active upon binding to calmodulin and calcium, functioning as a chaperone for BCL2 and targeting it to the mitochondria.
• The FKBP8/BCL2/calmodulin/calcium complex modulates BCL2 phosphorylation and likely interferes with its binding to pro-apoptotic targets, thus playing a role in apoptosis regulation.
• FKBP8 is involved in the inhibition of viral infection by influenza A viruses (IAV). Related references: PMID:28169297
• It is localized to the mitochondrial membrane, consistent with its role in mitochondrial apoptosis and protein trafficking.
• The protein contains TPR repeat domains and a transmembrane helix, and is subject to post‑translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin‑like conjugation.
• FKBP8 is widely expressed, with highest levels in the brain and retina, suggesting tissue‑specific regulatory functions in neuronal and retinal cells.

Experimental Guidance and Technical Tips

• The immunogen covers a central region (aa 60–270) of human FKBP38/FKBP8, which may enable detection of both full‑length and potential isoforms; always validate specificity in your sample system.
• For Western blot, expect a major band around 45 kDa; consider using whole‑cell lysates or mitochondrial fractions for optimal detection.
• In immunoprecipitation experiments, the mitochondrial membrane localization may require optimized lysis conditions (e.g., mild detergents) to preserve native interactions.
• For immunofluorescence/ICC, co‑staining with a mitochondrial marker (e.g., MitoTracker or anti‑TOM20) is recommended to confirm the expected subcellular distribution.
• ELISA applications should be validated with a suitable coating antigen and appropriate controls, as polyclonal antibodies may exhibit lot‑to‑lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material sourcing, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focused on apoptosis, viral infection, or mitochondrial biology, we provide reliable access to high‑quality anti‑FKBP38/FKBP8 reagents and can assist in sourcing rare‑target materials to accelerate your assay development.