Anti-FKBP38/FKBP8 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q14318

Core Product Specifications and Parameters

Biological Background: FKBP38/FKBP8 Function and Localization

• FKBP8, also known as FKBP38, hFKBP38, FKBP-38, FK506-binding protein 8, FKBPR38, or Rotamase, is a peptidyl-prolyl cis-trans isomerase (PPiase) belonging to the immunophilin family. It contains TPR repeats and a putative transmembrane helix.
• The enzyme is constitutively inactive as a PPiase and becomes active upon binding to calmodulin and calcium. It functions as a chaperone for BCL2, targeting BCL2 to mitochondria and modulating its phosphorylation state, thereby likely regulating apoptosis.
• FKBP8 plays a role in inhibiting infection by influenza A viruses (IAV). Related references: PMID:28169297
• The protein localizes to mitochondrial membranes and is an integral membrane protein.
• FKBP8 is subject to post-translational modifications, including phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation.
• Alternative splicing produces multiple isoforms of FKBP8.
• It is widely expressed in tissues, with highest levels in the brain, and is particularly abundant in the retina.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human FKBP8; consider validating specificity in knockout or knockdown systems.
• For Western blotting, the predicted molecular weight is 45 kDa; choose a gel percentage of 10-12% for optimal resolution.
• In immunofluorescence/ICC, FKBP8 localizes to mitochondria; co-staining with a mitochondrial marker is recommended.
• ELISA can be used for quantitative detection; empirical optimization of coating antigen and antibody dilutions is advised.
• As a recombinant monoclonal antibody, batch-to-batch consistency is expected, reducing experimental variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this FKBP8 clone, and bulk ancillary reagents. With expertise in rare target sourcing, we support projects from concept to clinic in areas such as apoptosis and mitochondrial research. Our integrated supply chain ensures reliable procurement of critical reagents for your assay development needs.