Anti-FKBP3 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q00688

Core Product Specifications and Parameters

Biological Background: FKBP3 Function and Localization

• FKBP3 encodes a 25 kDa FK506-binding protein, also known as FKBP25 or FKBP-3, and is a member of the immunophilin family.
• It functions as a peptidyl-prolyl cis-trans isomerase (PPIase) or rotamase, accelerating the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds.
• FKBP3 binds to the immunosuppressants FK506 and rapamycin, thereby modulating T-cell proliferation and distinct cytoplasmic signal transduction pathways.
• The protein is predominantly localized to the nucleus, as confirmed by subcellular localization studies and proteomics data.
• Post-translational modifications include phosphorylation and acetylation, which may regulate its activity and interactions.
• Its three-dimensional structure has been solved, providing insights into its catalytic and binding mechanisms.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, consider using reducing SDS-PAGE and PVDF membrane transfer; expect a band near 25 kDa.
• In immunoprecipitation (IP) experiments, validate antibody–protein complex recovery under native conditions and confirm with appropriate controls.
• For ELISA, test against recombinant FKBP3 protein or relevant biological samples to determine optimal antibody dilution.
• As a polyclonal antibody, evaluate lot-to-lot consistency if used in long-term studies or quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-FKBP3 polyclonal antibody supports research into immunophilin-mediated signaling and protein folding mechanisms. For your IVD development needs, CamelBio offers optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing services.