Anti-FKBP2 Rabbit Polyclonal Antibody for WB, ELISA - P26885

Core Product Specifications and Parameters

Biological Background: FKBP2 Function and Localization

• FKBP2 (also known as FKBP13, PPIase, or immunophilin FKBP13) is a peptidyl-prolyl cis-trans isomerase belonging to the FK506-binding protein family.
• It functions as a rotamase, catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, thereby accelerating protein folding.
• The protein is localized to the endoplasmic reticulum membrane, as indicated by its subcellular location and membrane keyword.
• FKBP2 shows tissue-specific expression, with high levels detected in T-cells and thymus.
• It is an immunophilin capable of binding the immunosuppressant FK506, linking it to immune regulation pathways.
• The protein has a signal peptide and a molecular weight of approximately 15.6 kDa (UniProt) or 16 kDa as observed experimentally.
• Proteomics identification confirms its presence in human samples, and its 3D structure has been solved (protein data bank).

Experimental Guidance and Technical Tips

• For Western blot, validate the antibody using protein extracts from human T-cell lines or mouse thymus tissue, which naturally express FKBP2. A band near 16 kDa is expected, though post-translational modifications or dimerization may alter migration.
• The immunogen (recombinant protein encompassing amino acids 22-142) covers the mature sequence, so the antibody can detect both precursor and processed forms.
• In ELISA applications, use the recombinant FKBP2 immunogen protein as a standard for quantification and to confirm assay specificity. Optimize coating buffer and blocking conditions.
• As a polyclonal antibody, consider lot-specific validation for reproducibility in quantitative assays. Cross-reactivity with mouse FKBP2 is indicated; sequence alignment with other FKBP family members can inform potential off-target binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated procurement solutions for IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents for immunophilin and protein folding research. Our portfolio features consistent supply of FKBP2-targeting reagents like this polyclonal antibody, alongside complementary products for ELISA and Western blot workflows. For specialized or hard-to-find targets, CamelBio’s sourcing expertise ensures efficient access to rare raw materials, supporting your diagnostic development from concept to clinic.