Anti-FKBP1B Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P68106

Core Product Specifications and Parameters

Biological Background: FKBP1B Function and Localization

• FKBP1B is a 12.6 kDa peptidyl-prolyl cis-trans isomerase (PPIase), also known as FKBP12.6, immunophilin FKBP12.6, or rotamase, encoded by the FKBP1B gene.
• It functions as an immunophilin that binds the immunosuppressive drugs FK506 and rapamycin, potentially contributing to their immunosuppressive and toxic effects.
• As a PPIase, it catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, thereby accelerating protein folding.
• Subcellularly, FKBP1B localizes to the cytoplasm and the sarcoplasmic reticulum.
• At the protein level, FKBP1B is detected in heart muscle; its isoforms are ubiquitously expressed with highest levels in the brain and thymus.
• Keywords: This protein is characterized by 3D-structure, alternative splicing, isomerase and rotamase activity, and is a component of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–80 of human FKBP1B (NP_473374.1). This N-terminal region may allow detection of multiple isoforms if the epitope is conserved; verify with specific samples.
• For Western blot (WB), the predicted molecular weight is 12 kDa. Using a 15% SDS-PAGE gel and careful transfer conditions (e.g., low-molecular-weight markers, wet transfer) may improve protein retention and detection.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider testing heart muscle and brain tissue where FKBP1B expression is reported to be abundant. Include positive and negative controls to validate staining specificity.
• The antibody is polyclonal; thus, it may exhibit higher sensitivity in ELISA. Confirm specificity via peptide competition or by antigen downregulation in model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, monoclonal antibodies, and ancillary reagents. For projects involving immunophilin biology, FKBP family proteins, or immunosuppressive drug mechanisms, CamelBio can source rare-target antibodies and bulk reagents to support your development pipeline from discovery to validation.