Anti-FKBP12 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P62942

Core Product Specifications and Parameters

Biological Background: FKBP12 Function and Localization

• FKBP12, also known as FKBP1A, FKBP1, or calstabin-1, is a 12 kDa FK506-binding protein that functions as a peptidyl-prolyl cis-trans isomerase (PPIase), accelerating protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
• It plays a key role in TGF-beta signaling by maintaining the type I receptor TGFBR1 in an inactive conformation in the absence of ligand, thereby preventing premature receptor activation.
• FKBP12 recruits SMAD7 to the activin receptor ACVR1B, inhibiting the association of SMAD2 and SMAD3 with the receptor complex and thus blocking activin signal transduction.
• The protein may modulate the activity of the ryanodine receptor (RYR1) calcium release channel, implicating it in calcium homeostasis.
• Subcellularly, FKBP12 is primarily localized in the cytoplasm and cytosol, and is also associated with the sarcoplasmic reticulum membrane.
• According to UniProt, FKBP12 undergoes acetylation and has been identified through direct protein sequencing and proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant protein spanning amino acids 2-108 of human FKBP12, encompassing nearly the full-length protein. This design may facilitate recognition of the native protein in various applications.
• For Western blot (WB) analysis, expect a band at approximately 12 kDa. Optimize loading and detection conditions, and include appropriate positive and negative controls.
• In IHC-P (immunohistochemistry of paraffin-embedded tissues), antigen retrieval may be required. Titrate the antibody to determine the optimal working concentration and validate with known positive and negative tissue samples.
• The antibody is reported to cross-react with mouse and rat FKBP12, making it suitable for studies in rodent models. However, cross-reactivity should be confirmed in the specific experimental system.
• For ELISA, consider using the antibody as a capture or detection reagent after testing for compatibility with the antigen and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes by providing a one-stop sourcing platform for IVD raw materials, including validated antibodies, ancillary reagents, and custom procurement services. This anti-FKBP12 polyclonal antibody is part of our extensive catalog for research in signal transduction, protein folding, and related areas. Whether you need bulk quantities, optimized antibody pairs, or rare target reagents, our team can assist in streamlining your supply chain from concept to clinic.