Anti-FKBP10 Rabbit PolymAb® for WB, IF/ICC, ELISA - Q96AY3

Core Product Specifications and Parameters

Biological Background: FKBP10 Function and Localization

• FKBP10 encodes a 65 kDa FK506-binding protein (FKBP65), a peptidyl-prolyl cis-trans isomerase (PPIase) that accelerates protein folding by catalyzing the isomerization of proline peptide bonds.
• It belongs to the FKBP immunophilin family and contains multiple FKBP-type peptidyl-prolyl cis-trans isomerase domains.
• FKBP10 is localized to the endoplasmic reticulum lumen, where it functions as a molecular chaperone involved in collagen biosynthesis and protein quality control.
• The protein is a glycoprotein and calcium-binding metalloprotein, with post-translational modifications including phosphorylation (Keywords: Glycoprotein, Calcium, Metal-binding, Phosphoprotein).
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity.
• Mutations in FKBP10 are associated with osteogenesis imperfecta, a genetic disorder characterized by bone fragility and connective tissue abnormalities.

Experimental Guidance and Technical Tips

• The antibody was raised against recombinant protein; it may recognize both denatured and native FKBP10, suitable for western blotting, immunofluorescence, and ELISA.
• For western blotting, a band at approximately 64 kDa is expected; consider loading appropriate molecular weight markers and using a suitable blocking buffer.
• When performing immunofluorescence, permeabilization is required to detect this endoplasmic reticulum-luminal protein; co-stain with an ER marker to confirm localization.
• Validation in cell lines or tissues expressing FKBP10, such as osteoblasts or fibroblasts, is recommended to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies involving FKBP10 and bone-related disorders, our portfolio includes optimized reagents to support your research and diagnostic development from concept to clinic.