Anti-FKBP10 Rabbit Polyclonal Antibody for WB - Q96AY3

Core Product Specifications and Parameters

Biological Background: FKBP10 Function and Localization

• FKBP10, also known as FKBP65, is a peptidyl-prolyl cis-trans isomerase (PPIase) that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, accelerating protein folding during synthesis.
• It belongs to the immunophilin family of FK506-binding proteins (FKBPs) and possesses rotamase activity.
• FKBP10 is localized to the endoplasmic reticulum lumen, as indicated by its signal peptide and subcellular localization data.
• The protein contains calcium-binding domains, suggesting regulation by calcium levels.
• It is a glycoprotein and undergoes phosphorylation, which may modulate its activity or interactions.
• Mutations in FKBP10 are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility.
• FKBP10 is subject to alternative splicing, potentially generating multiple isoforms with distinct functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 303–582, a region encompassing the FKBP-type peptidyl-prolyl cis-trans isomerase domains. For Western blot, consider using whole-cell lysates from human or mouse cell lines with high endoplasmic reticulum content. Since the antibody is KO validated, lysates from FKBP10 knockout cells or tissues can serve as a valuable negative control to confirm specificity.
• For ELISA, the antibody may detect both native and recombinant FKBP10; optimization of coating conditions and detection format is recommended.
• The predicted molecular weight is 64 kDa, but due to glycosylation and other post-translational modifications, the observed band may shift slightly. Always include appropriate molecular weight markers and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials, covering every stage from concept to clinic. This anti-FKBP10 antibody supports research into connective tissue disorders and protein folding diseases, offering a validated tool for development pipelines. Beyond individual antibodies, CamelBio supplies optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and specialized raw-material sourcing for rare targets—all designed to streamline your diagnostic development workflow.