Anti-FKBP10 Monoclonal Antibody for WB, IF/ICC, ELISA - Q96AY3

Core Product Specifications and Parameters

Biological Background: FKBP10 Function and Localization

• FKBP10, also known as FKBP65, is a peptidyl-prolyl cis-trans isomerase (PPIase) that catalyzes the cis-trans isomerization of proline imidic peptide bonds, accelerating protein folding during synthesis.
• It belongs to the FK506-binding protein (FKBP) family of immunophilins and contains multiple FKBP-type peptidyl-prolyl cis-trans isomerase domains.
• FKBP10 localizes to the endoplasmic reticulum (ER) lumen, where it likely functions in protein folding and quality control.
• Alternative splicing produces multiple isoforms; the full-length protein has a predicted molecular weight of ~64 kDa, while a shorter isoform migrates at ~39 kDa.
• The protein is glycosylated and phosphorylated, and contains calcium-binding motifs, consistent with its ER localization and role in folding of secretory and membrane proteins.
• Mutations in FKBP10 are associated with osteogenesis imperfecta (types XI and VI) and Bruck syndrome, underscoring its importance in collagen biosynthesis and bone development.
• It is also designated as an immunophilin, binding immunosuppressants like FK506 and rapamycin, though its physiological role in immune modulation is unclear.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 303-582 of human FKBP10, covering a region common to both the long and short isoforms; therefore, the antibody may recognize both isoforms in western blotting.
• For western blotting, expect major bands at ~64 kDa (full-length) and ~39 kDa (shorter isoform) in human cell lysates; use appropriate gel percentages for resolution.
• For immunofluorescence/ICC, FKBP10 localizes to the ER, yielding a reticular cytoplasmic staining pattern; co-staining with ER markers (e.g., calreticulin or PDI) can confirm specificity.
• For ELISA, use as a capture or detection antibody against recombinant FKBP10 protein or cell lysates; validate against known positive and negative controls to ensure sensitivity.
• Since FKBP10 is a low-abundance protein in many cell types, consider using enrichment strategies or high-sensitivity detection methods for assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, serving diagnostic manufacturers, clinical laboratories, and research institutions with a comprehensive portfolio from concept to clinic. Our FKBP10 antibody supports research into protein folding, ER stress, and bone-related disorders such as osteogenesis imperfecta. We offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development and manufacturing workflows.