Anti-FIP1L1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q6UN15

Core Product Specifications and Parameters

Biological Background: FIP1L1 Function and Localization

• FIP1L1 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, playing a key role in pre-mRNA 3'-end formation by recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase to mediate cleavage and poly(A) addition. Related references: PMID:29262316, PMID:30385546
• It binds to U-rich RNA sequence elements surrounding the poly(A) site and may act to tether poly(A) polymerase to the CPSF complex, contributing to poly(A) site recognition and stimulation of poly(A) addition. Related references: PMID:29262316, PMID:30385546
• FIP1L1 localizes to the nucleus, consistent with its role in mRNA 3'-end processing.
• The protein undergoes phosphorylation, as indicated by the phosphoprotein keyword, suggesting post-translational regulation.
• Alternative splicing of FIP1L1 generates multiple isoforms.
• FIP1L1 contains RNA-binding domains and has been identified through large-scale proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-300) of human FIP1L1, which may contribute to cross-reactivity with mouse and rat orthologs due to sequence conservation.
• For Western blotting (WB), the predicted molecular weight is ~67 kDa; consider using appropriate positive controls such as HeLa or HEK293 cell lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval methods and the nuclear localization of FIP1L1 for signal interpretation.
• For ELISA, use the antibody as a capture or detection reagent, with optimization of coating concentration and blocking conditions.
• As a polyclonal antibody, batch-to-batch variability may exist; validate performance in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on mRNA polyadenylation and pre-mRNA processing, our anti-FIP1L1 polyclonal antibody provides a reliable tool for multiple applications. Our supply chain and technical consulting services support diagnostic manufacturers and laboratories from concept to clinic.