Anti-FILIP1L Polyclonal Antibody for WB, ELISA - Q4L180

Core Product Specifications and Parameters

Biological Background: FILIP1L Function and Localization

• FILIP1L (Filamin A-interacting protein 1-like), also known as DOC1, GIP90, and GIP130, is encoded by the FILIP1L gene. Multiple isoforms exist due to alternative splicing, including 90 kDa and 130 kDa variants.
• Acts as a regulator of antiangiogenic activity in endothelial cells; overexpression inhibits cell proliferation and migration and increases apoptosis.
• When expressed in tumor-associated vasculature, FILIP1L inhibits melanoma growth.
• Subcellular localization includes the cytoplasm, membrane, and nucleus.
• Expressed in endothelial cells, colon, and ovarian epithelial cells. Notably down-regulated in ovarian cancer and strongly expressed in colon cancer stroma and vasculature.
• Classified as a phosphoprotein, suggesting regulation by phosphorylation.
• Contains coiled coil domains and has been identified through proteomics.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–200) covers the N-terminal region; consider that alternative isoforms lacking this region may not be detected.
• For western blotting, a band around 130 kDa is expected based on the full-length protein; smaller bands may correspond to isoforms or cleavage products.
• Validate antibody specificity in the relevant sample system using positive and negative controls (e.g., tissues with known FILIP1L expression).
• For ELISA applications, the recombinant immunogen can serve as a positive control for assay development.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio offers this anti-FILIP1L polyclonal antibody along with a comprehensive portfolio of antigens, antibodies, and ancillary reagents for angiogenesis and cancer research. Our supply capabilities extend to validated antibody pairs, bulk quantities, and customized rare-target sourcing to streamline your diagnostic development from concept to clinic.