Anti-Filamin-B (FLNB) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75369

Core Product Specifications and Parameters

Biological Background: Filamin-B Function and Localization

• Filamin-B (FLNB) is an actin-binding protein that crosslinks actin filaments and connects the cell membrane to the cytoskeleton.
• It promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins, anchoring various transmembrane proteins to the actin cytoskeleton.
• Interaction with filamin A (FLNA) may facilitate neuroblast migration from the ventricular zone to the cortical plate.
• Various isoforms (1–6) exist via alternative splicing; isoform 6 has been shown to accelerate muscle differentiation in vitro.
• Subcellular localization includes cytoplasm, cell cortex, stress fibers, and Z-lines of sarcomeres in muscle cells.
• Ubiquitously expressed with isoform-specific distribution: isoform 1 is predominant in many tissues, while others are enriched in heart, skeletal muscle, and leukocytes.
• Keywords: Acetylation, Actin-binding, Alternative splicing, Differentiation, Isopeptide bond, Myogenesis, Phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1686–1785 of human FLNB; consider this region when designing validation experiments or assessing isoform recognition.
• For WB, the predicted molecular weight is 278 kDa; use a low-percentage SDS-PAGE gel and extended transfer times to ensure efficient blotting of this high-molecular-weight protein.
• The antibody is applicable for IHC-P, IF/ICC, and ELISA; we recommend titrating the antibody and including appropriate positive and negative controls for each assay.
• Cross-reactivity with mouse and rat is reported based on sequence homology; verify antibody specificity in your target species samples if precise quantification is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw-material sourcing, offering validated antibodies and ancillary reagents to support your research and diagnostic development. Our anti-FLNB polyclonal antibody exemplifies our commitment to providing high-quality tools for cytoskeleton and cell migration studies. Explore our one-stop supply chain for bulk orders, technical consultation, and customization to accelerate your project from concept to clinic.