Anti-Filamin A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P21333

Core Product Specifications and Parameters

Biological Background: Filamin A Function and Localization

• Filamin A (FLNA), also known as actin-binding protein 280 (ABP-280) or alpha-filamin, is a large dimeric actin-crosslinking protein encoded by the FLNA gene.
• It promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins, providing mechanical stability to the cortical cytoskeleton.
• Serves as a scaffold for a wide range of cytoplasmic signaling proteins, anchoring various transmembrane proteins to the actin cytoskeleton and regulating intracellular trafficking (e.g., of furin).
• Plays a critical role in cell-cell contacts and adherens junctions during the development of the vasculature, heart, and brain.
• Involved in axon guidance; required for growth cone collapse induced by SEMA3A-mediated stimulation of neurons. Related references: PMID:25358863
• Subcellularly localized to the cytoplasm, cell cortex, cytoskeleton, growth cones, and podosomes; tissue expression is ubiquitous.
• Post-translationally modified by acetylation, phosphorylation, and ubiquitination; associated with cilium biogenesis and is a reference proteome component.
• Keywords include ‘Disease variant’ and ‘Deafness’, indicating that mutations in FLNA are linked to a spectrum of congenital disorders with skeletal, cardiovascular, and auditory manifestations.

Experimental Guidance and Technical Tips

• The immunogen spans a C-terminal region (aa 2348–2647) that is well-conserved; antibodies may recognize major FLNA isoforms. Verify isoform specificity if needed.
• For Western blotting, anticipate a band at approximately 281 kDa. Use low-percentage SDS-PAGE gels and extended transfer times to resolve this high-molecular-weight protein.
• For IHC-P and IF/ICC, consider optimizing antigen retrieval conditions and validate staining in appropriate positive-control tissues or cell lines.
• For ELISA, pair this antibody with a suitable capture or detection antibody after establishing optimal dilution ranges.
• Cross-reactivity is reported for human, mouse, and rat; confirm cross-species reactivity in your specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and life science innovators with comprehensive access to IVD raw materials, including validated Filamin A antibodies for cytoskeleton and cell adhesion research. As a one-stop sourcing partner, we offer optimized polyclonal and monoclonal antibodies, antibody pairs, and bulk ancillary reagents to streamline assay development from concept to clinic. Whether you need rare-target raw materials or technical consulting, our global supply network accelerates your path from research to diagnostic application.