Anti-Filamin A Monoclonal Antibody for WB, ELISA - P21333

Core Product Specifications and Parameters

Biological Background: Filamin A Function and Localization

• Filamin A (FLNA) is an actin-binding protein also known as ABP-280, alpha-filamin, endothelial actin-binding protein, filamin-1, or non-muscle filamin. It is encoded by the FLNA gene in humans and has a predicted molecular weight of approximately 281 kDa.
• It promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins, serving as a key organizer of the cortical actin cytoskeleton.
• FLNA anchors various transmembrane proteins to the actin cytoskeleton and acts as a scaffold for a wide range of cytoplasmic signaling proteins, facilitating signal transduction.
• It is involved in axon guidance and required for SEMA3A-mediated growth cone collapse in neurons, contributing to proper neuronal network formation (PubMed:25358863). Related references: PMID:25358863
• Subcellularly, Filamin A localizes to the cytoplasm, cell cortex, actin cytoskeleton, perikaryon, growth cones, and podosomes, reflecting its dynamic roles in cell architecture and motility.
• It is ubiquitously expressed across human tissues, consistent with its fundamental cytoskeletal functions.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation; mutations in FLNA are associated with diseases such as deafness and various developmental disorders (as indicated by disease variant keywords).

Experimental Guidance and Technical Tips

• The immunogen for this rabbit monoclonal antibody is a synthetic peptide from the C‑terminal region (amino acids 2548‑2647) of human Filamin A. This design may provide good specificity for Filamin A and potential reactivity with isoforms containing this epitope.
• For Western blotting, consider using a low‑percentage SDS‑PAGE gel (e.g., 6% or gradient gel) to resolve the ~281 kDa protein, and optimize transfer conditions for high‑molecular‑weight proteins (e.g., using wet transfer with reduced methanol).
• For ELISA, standard direct or indirect protocols can be employed; titrate the antibody to determine the optimal working concentration for your specific assay format.
• The antibody is reported to cross‑react with mouse, so validation in mouse‑derived samples is recommended when applicable.
• Always include appropriate positive (e.g., HeLa, HEK293 lysates) and negative controls in your experiments to confirm specificity.

CamelBio: Your One‑Stop Sourcing Bridge

As a trusted partner for diagnostic manufacturers and research institutions, CamelBio provides comprehensive access to IVD raw materials including validated antibodies, antigen pairs, and ancillary reagents. Our Filamin A rabbit monoclonal antibody exemplifies our commitment to supporting cytoskeleton and signaling pathway research with high‑quality, reproducible tools. From single antibodies to bulk custom procurement, we streamline your sourcing journey from concept to clinic.