Anti-Filaggrin Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P20930

Core Product Specifications and Parameters

Biological Background: Filaggrin Function and Localization

• Filaggrin is a structural protein essential for aggregating keratin intermediate filaments during terminal epidermal differentiation, promoting disulfide-bond formation for cornified envelope integrity.
• The protein is initially expressed as profilaggrin, a large precursor that is proteolytically processed into active filaggrin monomers within the keratinocyte.
• Filaggrin is predominantly localized to cytoplasmic keratohyalin granules in the stratum granulosum layer of the epidermis.
• In addition to skin, FLG is expressed in thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland, and trachea, but not in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland.
• Keywords associated with filaggrin include 3D-structure, calcium-binding, citrullination, coiled coil, developmental protein, ichthyosis, metal-binding, phosphoprotein, and repeat regions, highlighting its multifaceted role in skin biology and disease.
• Loss-of-function mutations in FLG are a major genetic risk factor for atopic dermatitis and ichthyosis vulgaris, underscoring its critical barrier function.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit monoclonal antibody corresponds to amino acids 198-288 of human filaggrin (NP_002007.1), a region that may be conserved in processed filaggrin fragments; consider this when designing experiments.
• For immunofluorescence (IF-P), filaggrin is expected to localize to cytoplasmic keratohyalin granules in differentiated keratinocytes; optimize permeabilization and antigen retrieval conditions for stratum granulosum-specific staining.
• In immunohistochemistry (IHC-P) of skin sections, filaggrin staining is typically prominent in the granular layer; validate antibody specificity with appropriate positive and negative tissue controls.
• For ELISA, the recombinant immunogen can serve as a positive control; ensure proper coating concentration and antibody dilution for optimal signal-to-noise ratio.
• Given the large size and repetitive nature of filaggrin, consider potential cross-reactivity with other S100 fused-type proteins; verify in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated monoclonal antibodies like this anti-Filaggrin rabbit mAb. In addition to filaggrin-related reagents for dermatological research, we offer a wide portfolio of antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio ensures reliable supply and technical support for your assay development needs.