Anti-Fibronectin Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P02751

Core Product Specifications and Parameters

Biological Background: Fibronectin Function and Localization

• Fibronectins are high-molecular-weight glycoproteins that bind to cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. They play essential roles in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Related references: PMID:3024962 PMID:3593230 PMID:3900070

• Fibronectin acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Related references: PMID:34089617

• The anastellin fragment binds fibronectin and induces superfibronectin formation, which exhibits enhanced adhesive properties and inhibits tumor growth, angiogenesis, and metastasis.

• Fibronectin secreted by contracting muscle induces liver autophagy and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.

• Fibronectin is involved in osteoblast compaction through fibrillogenesis and regulates type I collagen deposition, essential for bone mineralization.

• Subcellular location: Secreted into the extracellular space and deposited in the extracellular matrix.

• Tissue specificity: Plasma fibronectin (soluble dimer) is secreted by hepatocytes; cellular fibronectin (dimeric or multimeric) is produced by fibroblasts, epithelial cells, and deposited as fibrils in the ECM. Expressed in retina inner limiting membrane and around blood vessels. Ugl-Y1, Ugl-Y2 and Ugl-Y3 isoforms are found in urine.

• Post-translational modifications include phosphorylation, sulfation, and disulfide bond formation; the protein contains heparin-binding domains and is a glycoprotein.

Experimental Guidance and Technical Tips

• The recombinant full-length immunogen suggests the antibody may detect multiple fibronectin isoforms, including plasma and cellular forms. Validate specificity in relevant biological samples.
• For WB, note the predicted molecular weight of ~272 kDa. Use a low percentage acrylamide gel (e.g., 6% or gradient gel) and ensure proper transfer of large proteins.
• For IF/ICC, optimize fixation and permeabilization steps; fibronectin is an extracellular matrix protein, so permeabilization may require stronger detergents or longer incubation.
• ELISA may be applicable for detecting fibronectin in serum, plasma, or cell supernatants. Perform cross-reactivity testing if working with mouse samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio supports your fibronectin-related research and diagnostic development with reliable supply chain solutions.