Anti-Fibronectin Polyclonal Antibody for WB, IF/ICC, ELISA - P11276

Core Product Specifications and Parameters

Biological Background: Fibronectin Function and Localization

• Fibronectin is a high-molecular-weight glycoprotein of the extracellular matrix (ECM) that binds cell surfaces and various compounds, including collagen, fibrin, heparin, DNA, and actin.
• It plays critical roles in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
• Fibronectin participates in osteoblast compaction and regulates type I collagen deposition, essential for osteoblast mineralization. Related references: PMID:21768292
• Acts as a ligand for the Lilrb4a receptor, inhibiting Fcgr1/CD64-mediated monocyte activation. Related references: PMID:34089617
• Secreted by contracting muscle, fibronectin induces liver autophagy and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
• Fibronectin self-assembles into fibrils; anastellin and superfibronectin inhibit tumor growth, angiogenesis, and metastasis.
• Alternative splicing generates multiple isoforms; soluble plasma fibronectin is produced by hepatocytes, while insoluble cellular fibronectin is deposited by fibroblasts and epithelial cells.
• Subcellular location: Secreted into the extracellular space and ECM; tissue expression includes retina inner limiting membrane and around blood vessels.

Experimental Guidance and Technical Tips

• For Western blot, a band at ~273 kDa is expected; consider using low-percentage gels and prolonged transfer times due to the large size.
• Fibronectin may form disulfide-linked dimers; reducing conditions are recommended for monomeric band detection.
• In immunofluorescence (IF/ICC), permeabilization steps should be optimized to detect extracellular matrix deposits; cellular fibronectin often appears as a fibrillar network.
• For ELISA, this polyclonal antibody can be used as capture or detection reagent; validate pairing with monoclonal antibodies for sandwich assays.
• The immunogen maps to the C-terminal region (aa 2205–2477), conserved across human, mouse, and rat, supporting cross-reactivity; always confirm reactivity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

Fibronectin is a pivotal target in ECM biology, fibrosis, and wound healing research. CamelBio offers end-to-end sourcing solutions for diagnostic manufacturers and research laboratories, from validated antibody pairs and high-quality polyclonal/monoclonal antibodies to bulk ancillary reagents and rare-target raw materials. Whether you need consistent immunoreagents or technical consulting for assay development, CamelBio bridges the gap from concept to clinical application.