Anti-Fibronectin Polyclonal Antibody for WB, IF/ICC, ELISA - P02751

Core Product Specifications and Parameters

Biological Background: Fibronectin Function and Localization

• Fibronectin is a secreted, extracellular matrix glycoprotein that binds cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin, playing essential roles in cell adhesion, motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070). Related references: PMID:3024962, PMID:3593230, PMID:3900070
• It participates in osteoblast compaction and mineralization through fibrillogenesis and regulates type I collagen deposition by osteoblasts (By similarity).
• Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). Related references: PMID:34089617
• The protein exists in soluble plasma form secreted by hepatocytes and cellular form deposited as extracellular matrix fibrils by fibroblasts, epithelial, and other cell types (PubMed:29777959, PubMed:17614963). Related references: PMID:29777959, PMID:17614963
• It undergoes alternative splicing, contains isopeptide bonds, and is extensively post-translationally modified (glycosylation, phosphorylation, sulfation) as indicated by keywords including 'Acute phase', 'Disulfide bond', 'Glycoprotein', 'Phosphoprotein'.
• A fragment called anastellin induces fibronectin polymerization into superfibronectin, which inhibits tumor growth, angiogenesis, and metastasis by activating p38 MAPK and inhibiting lysophospholipid signaling.
• Secreted by contracting muscle, it induces liver autophagy and systemic insulin sensitization through hepatic ITGA5:ITGB1 integrin receptor signaling.
• Tissue specificity includes expression in retinal inner limiting membrane and around blood vessels, and it is found in urine as distinct isoforms (Ugl-Y1, Ugl-Y2, Ugl-Y3) (PubMed:29777959, PubMed:17614963). Related references: PMID:29777959, PMID:17614963

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to amino acids 2169-2355 within the C-terminal region of human Fibronectin. For applications like WB and IF/ICC, consider titrating the antibody and validating in the relevant sample system to confirm specificity.
• Since the antigen sequence is conserved across human, mouse, and rat (as indicated by cross-reactivity), it may be suitable for cross-species analyses; however, verify performance in each species independently.
• For ELISA applications, ensure appropriate coating conditions and detection methods, and remember that plasma fibronectin is abundant; cellular fibronectin may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on cell adhesion, extracellular matrix biology, and related fields, CamelBio streamlines the sourcing of rare-target reagents and can supply custom antibody development and technical consultation, bridging the gap from concept to clinic. Our portfolio supports robust assay development and ensures quality raw material procurement for your diagnostic and research needs.