Anti-Fibroblast activation protein-α (FAP) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q12884

Core Product Specifications and Parameters

Biological Background: Fibroblast Activation Protein Alpha (FAP) Function and Localization

• FAP, also known as seprase and SIMP, is a cell-surface glycoprotein serine protease.
• Its endopeptidase and dipeptidyl peptidase activities target substrates like alpha-2-antiplasmin, SPRY2, and neuropeptides (NPY, PYY, substance P), with preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences.
  Related references: PMID:14751930, PMID:16223769, PMID:16410248, PMID:10347120, PMID:10593948, PMID:16175601, PMID:21314817
• FAP degrades gelatin and heat-denatured type I collagen, facilitating cell adhesion, migration, and invasion during tissue remodeling.
  Related references: PMID:10347120, PMID:10455171, PMID:12376466
• In tumors, FAP promotes glioma invasion by degrading brevican and enhances angiogenesis, collagen degradation, and immune evasion, supporting malignant progression.
• Subcellularly, FAP is found on the cell membrane, lamellipodia, invadopodia, and ruffles; it can also be secreted or present in the cytoplasm.
• Tissue expression is restricted to activated fibroblasts (e.g., wound healing, cancer stroma, cirrhotic liver), glioma cells, and adipose tissue, but absent in normal liver.
• The FAP gene produces at least two isoforms via alternative splicing, both expressed in melanoma, carcinoma, and fibroblast cell lines.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of FAP is ~88 kDa; use appropriate gel percentages (e.g., 8–10% SDS-PAGE) and confirm band specificity with relevant controls.
• In immunofluorescence/ICC, permeabilization steps are essential to detect membrane-localized FAP; validate staining in positive cell lines such as activated fibroblasts or cancer cells.
• For ELISA applications, this polyclonal antibody may serve as a capture or detection reagent; pairing optimization and appropriate blocking are recommended.
• Given the polyclonal nature and near-full-length immunogen, the antibody likely recognizes multiple epitopes, which can be advantageous for detecting variants across human, mouse, and rat.
• Verify cross-reactivity with mouse and rat samples using species-specific positive controls, as expression levels may vary.
• Consider that soluble FAP is present in some biological fluids, which could compete with membrane-bound forms in certain assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering antibodies, antigens, and ancillary reagents to support your assay development. For researchers and manufacturers focusing on fibrosis, cancer, or wound healing targets like FAP, we provide validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk reagent supply. From initial R&D to clinical applications, our one-stop service streamlines your procurement of high-quality diagnostic raw materials.