Anti-Fibrinogen alpha chain (FGA) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P02671

Core Product Specifications and Parameters

Biological Background: Fibrinogen alpha chain Function and Localization

• Fibrinogen alpha chain (FGA) is a component of the blood coagulation factor fibrinogen, together with beta (FGB) and gamma (FGG) chains. It is cleaved by thrombin to form fibrin monomers that polymerize into an insoluble fibrin clot, essential for hemostasis.
• Beyond clot formation, fibrin facilitates early wound repair by stabilizing lesions and guiding cell migration during re-epithelialization.
• Maternal fibrinogen is required for successful pregnancy, and fibrin deposition plays a protective role in infections by preventing IFNG-mediated hemorrhage and modulating innate and T-cell mediated immunity.
• FGA enhances SELP expression on activated platelets via an ITGB3-dependent pathway, contributing to platelet activity without being absolutely required for thrombus formation in vivo.
• The protein is secreted into blood plasma, where it circulates at high concentrations as a soluble precursor.
• Post-translational modifications include alternative splicing, disulfide bond formation, glycosylation, phosphorylation, hydroxylation, and isopeptide bond formation, reflecting structural and functional complexity.
• Mutations in FGA are associated with coagulation disorders and amyloidosis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 700-800, a C-terminal region. This may allow detection of both full-length FGA and certain splice isoforms; verify in relevant sample lysates.
• For Western blot, consider using standard SDS-PAGE conditions with reducing agents; the predicted molecular weight is approximately 95 kDa, but under reducing conditions, the fibrinogen chains dissociate into individual alpha, beta, and gamma chains.
• For IHC-P, antigen retrieval may be necessary. Optimize with citrate or EDTA buffer and validate in known positive human tissue (e.g., liver, placenta) and cross-reactive rat samples.
• In ELISA applications, the monoclonal nature of this antibody makes it suitable for both capture and detection; evaluate buffer conditions and coating concentrations for your specific assay format.
• Although cross-reactivity with rat is confirmed, always perform initial suitability testing for your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering antibodies, validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary reagents, and rare target raw-material procurement. This anti-FGA rabbit monoclonal antibody serves hemostasis and coagulation research, supporting diagnostic manufacturers, labs, and institutes from concept to clinic. Contact us for bulk orders, custom conjugations, or full supply chain solutions.